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Anti-CD61 (Phospho-Y785) Antibody

Catalog Number: orb393088

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb393088
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ITGB3 (Phospho-Y785)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Porcine, Rabbit, Rat, Sheep
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y785 of human CD61 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetITGB3
Entrez16416, 3690
UniProt IDO54890, P05106
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesGP3A; Integrin beta-3; Platelet membrane glycoprot
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CD61 (Phospho-Y785) Antibody

Western blot analysis of CD61 (Phospho-Y785) expression in U87MG (A), PC3 (B) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 110 kD)

Anti-CD61 (Phospho-Y785) Antibody

Immunofluorescent analysis of CD61 (Phospho-Y785) staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Anti-CD61 (Phospho-Y785) Antibody

Direct ELISA antibody dose-response curve using Anti-CD61 (Phospho-Y785) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.