Anti-CD59 [193-27]

• Catalog Number: orb758928
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD59
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 193-27
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgM
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD59
• UniProt ID: P13987
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: 1F5; 1F5 antigen; CD59 glycoprotein; 20 kDa homolo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-CD59 antibody 193-27. Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of 193-27 (orb758929, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 193-27 (orb758929) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758929, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.