Anti-CD53 [HD77]

• Catalog Number: orb758893
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD53
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HD77
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising mice with human leukemia cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD53
• UniProt ID: P19397
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TSPAN-25; Leukocyte surface antigen CD53; Tetraspa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD53 antibody HD77. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of HD77 (orb758893, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD53 antibody HD77. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version HD77 (orb758893) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758893, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD53 antibody HD77 Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (orb758893) at 0.1 µg/ml, 0.3 µg/ml and 0.5 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.