Anti-CD52 [YTH 34.5-G2b (Campath-1G)]

• Catalog Number: orb348851
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD52
• Target: CD52
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Primate
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Human peripheral mononuclear cells enriched for T-cells.
• UniProt ID: P31358
• Tested applications: ELISA, FC, IHC-Fr, IHC-P, WB
• Clone Number: YTH 34.5-G2b (Campath-1G)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDw52; Cambridge pathology 1 antigen; Epididymal s
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hale, G. et al. Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement Blood, 62, 873-882 (1983)

PubMed: 6349718

Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (orb348905, panel B) at a concentration of 0.25 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Western Blot using anti-CD52 antibody YTH 34.5-G2b (Campath-1G) Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (orb378215) at 1.5 µg/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. orb378215 successfully detected CD52 in mouse spleen samples.