CD49f Antibody (APC)

• Catalog Number: orb1806413
• Category: Antibodies
• Description: CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.
• Target: CD49f
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Conjugation: APC
• Reactivity: Bovine, Canine, Equine, Feline, Human, Mouse, Porcine, Primate, Rabbit, Sheep
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: mouse mammary tumor cells
• UniProt ID: P23229
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: GoH3
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: ITGA6, VLA6alpha, platelet gpIc, integrin subunit
• Note: For research use only
• Entrez: 3655
• Expiration Date: 12 months from date of receipt.

Separation of human CD49f positive thrombocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49f (GoH3) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49f (GoH3) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).