CD49e Antibody

SKU: orb1474522

Description

The CD49e Antibody is suitable for IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human Integrin alpha 5 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Integrin alpha 5
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FNRA; Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e

Western blot analysis of CD49e expression in K562 (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 150 kD)

Immunohistochemical analysis of CD49e staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.15). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P08648, P11688

NCBI Gene ID

16402, 3678