Anti-CD49e Antibody

• Catalog Number: orb1474522
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD49e
• Target: ITGA5
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: A synthetic peptide of human Integrin alpha 5
• UniProt ID: P08648, P11688
• Tested applications: IH, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FNRA; Integrin alpha-5; CD49 antigen-like family m
• Note: For research use only
• Entrez: 3678, 16402

Western blot analysis of CD49e expression in K562 (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 150 kD)

Immunohistochemical analysis of CD49e staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.15). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.