Anti-CD48 antibody(DM44), Rabbit mAb

• Catalog Number: orb757370
• Category: Antibodies
• Description: Anti-CD48 antibody(DM44), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM44
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD48
• UniProt ID: P09326
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD48, BCM1, SLAMF2, BLAST, BLAST1, MEM-102, TCT.1,
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Expi 293 cell line transfected with irrelevant protein (left) and human CD48 (right) were surface stained with Rabbit anti-CD48 monoclonal antibody 1 μg/ml (clone: DM44) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD48 monoclonal antibody (clone: DM44) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human CD48 mAb (orb757370). (A) orb757370 does not bind to 293T cells that do not express CD48. (B) A clear peak shift of orb757370 was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of orb757370 to CD48. Antibodies were incubated at 2 μg/mL.

Anti-CD48 antibody (SKU# orb757370) at 1/1000 dilution.Lane: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 28 kDa.Observed band size: 40 kDa