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Anti-CD47 (magrolimab biosimilar;IgG1) mAb

Catalog Number: orb1173650

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1173650
CategoryAntibodies
DescriptionAnti-CD47 (magrolimab biosimilar, IgG1) mAb
Species/HostHuman
ClonalityMonoclonal
Tested applicationsELISA, FC
ReactivityHuman
IsotypeIgG1
Antibody TypeBiosimilar Antibody
Dilution rangeELISA 1:5000-10000|Flow Cyt 1:100
Form/AppearancePowder
ConjugationUnconjugated
TargetCD47
UniProt IDQ08722
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCD47;MER6;IAP
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NoteFor research use only
Application notesLyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date12 months from date of receipt.
Anti-CD47 (magrolimab biosimilar;IgG1) mAb

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein orb689369 can bind Anti-CD47 (magrolimab biosimilar, IgG1) orb1173650 in a linear range of 3.2-16.0 ng/ml.

Anti-CD47 (magrolimab biosimilar;IgG1) mAb

Jurkat cell line were surface stained with anti-CD47 (orb1173650) (Blue histogram) and isotype control (Red histogram) 1 μg/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.

Anti-CD47 (magrolimab biosimilar;IgG1) mAb

Flow cytometry analysis of antigen binding of anti-human CD47 mAb (orb1173650). (A) orb1173650 does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of orb1173650 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of orb1173650 to CD47. Antibodies were incubated at 5 μg/mL.