Anti-CD47 [B6H12]

• Catalog Number: orb1821595
• Category: Antibodies
• Description: Anti-CD47 [B6H12]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B6H12
• Tested applications: Blocking, ELISA, FC, IP
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with purified placental RGD-binding proteins (PRBP).
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD47
• UniProt ID: Q08722
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IAP; MER6; OV-3; Leukocyte surface antigen CD47; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD47 antibody B6H12. Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of B6H12 (orb1821595, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells with Anti-CD47 B6H12. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ plus cover-slips stained with the chimeric rabbit IgG version of B6H12 (orb1821595) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821595, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-CD47 antibody B6H12. Human PBM cells lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of B6H12 (orb1821595) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.