CD45 Mouse Monoclonal Antibody (PE/Cy7)

SKU: orb1293559

Description

The CD45 Antibody-PE/Cy7 labled is suitable for FC, IF. It is a Monoclonal, PE/Cy7 conjugated antibody which raised against Native purified human CD45.. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: 10 μl / assay
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 9.4
• Immunogen: Native purified human CD45.
• Target: PTPRC
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: PE/Cy7

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575

NCBI Gene ID

5788