CD45 Antibody-PE/Cy5.5 labled

SKU: orb1293582

Description

The CD45 Antibody-PE/Cy5.5 labled is suitable for FC, IF. It is a Monoclonal, PE/Cy5.5 conjugated antibody which raised against Mouse thymus or spleen. Purification: The antibody was purified by affinity chromatography.

Images & Validation

• Tested Applications: FC, IF
• Dilution range: 10 μl / assay
• Reactivity: Mouse
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Clone No.: I3/2.3
• Immunogen: Mouse thymus or spleen
• Purification: The antibody was purified by affinity chromatography.

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Rat IgG2b lambda. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Disclaimer: For research use only

Alternative Names

CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45

Quick Database Links

UniProt

P06800

NCBI Gene ID

19264