Anti-CD44 (Indian blood group) [SAIC-01B-4D8]

• Catalog Number: orb1090001
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD44 (Indian blood group)
• Target: CD44 (Indian blood group)
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Peptide "TFIPVTSAK" derived from CD44, a hyaluronic acid receptor mediating cell-cell and cell-matrix interactions , conjugated to KLH.
• UniProt ID: P16070
• Tested applications: ELISA, WB
• Clone Number: SAIC-01B-4D8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LHR; MDU2; MDU3; MIC4; Extracellular matrix recept
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD44 (Indian blood group) antibody SAIC-01B-4D8. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-01B-4D8 (orb1090001, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-CD44 (Indian blood group) SAIC-01B-4D8. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of SAIC-01B-4D8 (orb1090001) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1090001, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.