Anti-CD44 [Hermes-1]

• Catalog Number: orb613886
• Category: Antibodies
• Description: Rat monoclonal antibody to CD44
• Target: CD44
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Bovine, Goat, Human, Mouse, Primate, Rabbit, Rat, Sheep
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).
• UniProt ID: P16070
• Tested applications: Blocking, ELISA, FC, ICC, IF, IHC, IP, NeA, WB
• Clone Number: Hermes-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDw44; Epican; Extracellular matrix receptor III;
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-CD44 antibody Hermes-1 Human activated T lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Hermes-1 (orb613885, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.