Anti-CD42c Antibody

• Catalog Number: orb213989
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GP1BB
• Target: GP1BB
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD42c. The exact sequence is proprietary.
• UniProt ID: Q9JJM7, P13224, P56400
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Platelet glycoprotein Ib beta chain antibody,
• Note: For research use only
• Entrez: 14724, 2812, 116727

Western blot analysis of CD42c expression in mouse testis (A), mouse heart (B), rat testis (C), rat heart (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)

Immunofluorescent analysis of CD42c staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).