Anti-CD40L [hu5c8 (Ruplizumab)]

• Catalog Number: orb348859
• Category: Antibodies
• Description: Human monoclonal antibody to CD40L
• Target: CD40L
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Human CD40L
• UniProt ID: P29965
• Tested applications: Blocking, FC, WB
• Clone Number: hu5c8 (Ruplizumab)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD154; CD40 ligand; tumour necrosis factor ligand
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Ruplizimab (orb348886, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Western Blot using anti-OX40L antibody. Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with orb348859 at 1 µg/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.