Anti-CD40L [AT161-10]

• Catalog Number: orb758775
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD40L
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AT161-10
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD40L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD154; CD40 ligand; CD-40L; CD-40 Ligand; CD40-L;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD40L antibody AT161-10. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of AT161-10 (orb758776, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood monocytes using anti-CD40L antibody AT161-10. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of AT161-10 (orb758776) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758776, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.