CD40 Ligand Biosimilar Antibody

• Catalog Number: orb1173657
• Category: Antibodies
• Description: Anti-CD40L (ruplizumab biosimilar) mAb
• Target: CD40 Ligand
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P29965
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000 Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD40LGCD154CD40LHIGM1IGMIMD3T-BAMTNFSF5TRAPgp39
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD40 Ligand Protein, hFc Tag (orb689443) can bind Anti-CD40 Ligand (ruplizumab biosimilar) mAb (orb1173657) in a linear range of 0.64-400 ng/ml. In order to specifically detect orb1173657, mouse anti-human Fab-specific antibody was used as detection antibody.

HEK293 cell line transfected with irrelevant protein (B) and human CD40L (A) were surface stained with Anti-CD40L mAb 1 μg/ml (ruplizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.