Anti-CD40 [FGK45]

• Catalog Number: orb1152478
• Category: Antibodies
• Description: Rat monoclonal antibody to CD40
• Target: CD40
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original rat IgG2a antibody was raised against the recombinant mouse CD40 fusion protein.
• UniProt ID: P27512
• Tested applications: FA, FC, WB
• Clone Number: FGK45
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tnfrsf5; Tumor necrosis factor receptor superfamil
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD40 antibody FGK45. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of FGK45 (orb1152479, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-CD40 FGK45. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of FGK45 (orb1152479) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152479, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-CD40 antibody FGK45. Mouse lymph node tissue lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FGK45 (orb1152479) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.