Anti-CD40 [5D12]

• Catalog Number: orb613924
• Category: Antibodies
• Description: Human monoclonal antibody to CD40
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 5D12
• Tested applications: Blocking, ELISA, FC, IHC, IP
• Reactivity: Human, Monkey
• Isotype: IgG4
• Immunogen: Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD40
• UniProt ID: P25942
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRS5;Tumor necrosis factor receptor superfamily
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody 5D12. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 5D12 (orb613925) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613925, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.