Anti-CD40 [3/23]

• Catalog Number: orb758828
• Category: Antibodies
• Description: Rat monoclonal antibody to CD40
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 3/23
• Tested applications: FC, IHC, IP
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising Lou rats with murine CD40-hγ1-Fc according to the protocol by Hasbold et al. (Eur J Immunol. 1994)
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD40
• UniProt ID: P27512
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRSF5; B-cell surface antigen CD40; Bp50; CD40;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD40 antibody 3/23. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3/23 (orb758827) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758827, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.