Anti-CD4 [YNB46.1.8 (Campath-9H)]

• Catalog Number: orb348926
• Category: Antibodies
• Description: Rat monoclonal antibody to CD4
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: YNB46.1.8 (Campath-9H)
• Tested applications: FC, IHC, IHC-Fr
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Rat T-cell line (NB2) transfected with the human CD4.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD4
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leu-3/T4; Cluster of differentiation 4; T-cell sur
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (orb348906, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of rabbit spleen using anti-CD4 antibody YNB46.1.8 (Campath-9H) Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (orb348926) was used to stain samples at a concentration of 5 µg/ml.

Western Blot using anti-CD4 antibody. Mouse thymus (lane 1) and mouse spleen (lane 2).