CD4/LEU3 Antibody

SKU: orb1805876

Description

Anti-CD4 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human, Monkey

Key Properties

• Clonality: Monoclonal
• Target: CD4 / LEU3
• Source/Expression System: CHO
• Molecular Weight: 147.36 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-CD4 Reference Antibody (ibalizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CD4 Reference Antibody (ibalizumab)is more than 95% ;determined by SEC-HPLC.

Human CD4 CHO cells were stained with Anti-CD4 Reference Antibody (ibalizumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC369=0.16 μg/mL.

Anti-CD4 Reference Antibody (ibalizumab) Pseudoviral inhibition was evaluated using Tzmbl.The IC50 was approximately 0.441 nM.

Anti-CD4 Reference Antibody (ibalizumab)Activation inhibition was evaluated using PBMC. The max induction fold was approximately 1.71.

Quick Database Links

Gene Symbol

CD4 / LEU3

UniProt

P01730