Anti-CD4 [CE9.1 (Clenoliximab)]

SKU: orb348872

Description

Human monoclonal antibody to CD4

Research Area

Immunology & Inflammation, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: Blocking, ELISA, FC, IHC
• Reactivity: Human, Monkey

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG4
• Clone No.: CE9.1 (Clenoliximab)
• Immunogen: Domain 1 of human CD4.
• Target: CD4
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

p55; T cell surface antign T4/Leu-3.

Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Clenoliximab (orb348883, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of guinea pig skin using anti-CD4 antibody CE9.1 (Clenoliximab) Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (orb348872) was used to stain samples at a concentration of 3 µg/ml.

Western Blot using anti-CD4 antibody CE9.1 (Clenoliximab) Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (orb348872) at 1.5 µg/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. orb348872 successfully detected CD4 in human thyroid tissue samples.

Quick Database Links

Gene Symbol

CD4

UniProt

P01730