CD3e Antibody-APC labled

SKU: orb1294129

Description

The CD3e Antibody-APC labled is suitable for FC, IF. It is a Monoclonal, APC conjugated antibody. Purification: The antibody was purified by affinity chromatography.

Images & Validation

• Tested Applications: FC, IF
• Dilution range: 10 μl / assay
• Reactivity: Mouse
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Hamster
• Clonality: Monoclonal
• Clone No.: 145-2C11
• Purification: The antibody was purified by affinity chromatography.

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Armenian Hamster IgG. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Disclaimer: For research use only

Alternative Names

T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e

Quick Database Links

UniProt

P22646

NCBI Gene ID

12501