Anti-CD3e [898H2-6-15]

• Catalog Number: orb1152491
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD3e
• Target: CD3e
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Porcine
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
• UniProt ID: Q7YRN2
• Tested applications: FC, IHC
• Clone Number: 898H2-6-15
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Leu4/T3; Cluster of Differentiation 3 epsilon chai
• Note: For research use only

Flow cytometry using the Anti-CD3e antibody 898H2-6-15. Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 898H2-6-15 (orb1152491, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of pig peripheral blood monocytes with anti-CD3e 898H2-6-15. Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 898H2-6-15 (orb1152491) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152491, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-CD3e antibody 898H2-6-15. Pig spleen(A) (1 µg/ml), pig thymus(B) (0.1 µg/ml) and lymph node(C) (0.1 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 898H2-6-15 (orb1152491) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.