Anti-CD37 [IPO-24 (ZC37-24)]

SKU: orb758922

Description

Rabbit monoclonal antibody to CD37

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: IPO-24 (ZC37-24)
• Immunogen: This antibody was raised by immunizing mice with spleen cells of a patient with hairy cell leukemia.
• Target: CD37
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tetraspanin-26; Leukocyte antigen CD37; tetraspanin; Tspan-26; Gp52-40

Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody IPO-24 (ZC37-24). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of IPO-24 (ZC37-24) (orb758922) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758922, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD37 antibody IPO-24 (ZC37-24) Daudi cell lysate(A), human spleen(B), and tonsil(C) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of IPO-24 (ZC37-24) (orb758922) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CD37

UniProt

P11049