Anti-CD363 (Phospho-T236) Antibody

• Catalog Number: orb393159
• Category: Antibodies
• Description: Rabbit polyclonal antibody to S1PR1 (Phospho-T236)
• Target: S1PR1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T236 of human CD363 protein. The exact sequence is proprietary.
• UniProt ID: P48303, O08530, P21453
• Tested applications: IF, WB
• Dilution range: WB: 1:500:1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1;
• Note: For research use only
• Entrez: 13609, 29733, 1901

Western blot analysis of CD363 (Phospho-T236) expression in HepG2 UV-treated (A), mouse brain (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)

Immunofluorescent analysis of CD363 (Phospho-T236) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.