Anti-CD33 antibody(DM77); Rabbit mAb

• Catalog Number: orb757402
• Category: Antibodies
• Description: Anti-CD33 antibody(DM77), Rabbit mAb
• Target: CD33
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• UniProt ID: P20138
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM77
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD33,SIGLEC3,gp67
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD33 protein, hFc-His tagged protein orb689399 can bind Rabbit anti-CD33 monoclonal antibody (clone: DM77) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human CD33 (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1 μg/ml (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human CD33 mAb (orb757402). (A) orb757402 does not bind to 293T cells that do not express CD33. (B) A clear peak shift of orb757402 was seen compared to the control when incubated with CD33-expressing THP-1 cells, indicating strong binding of orb757402 to CD33. Antibodies were incubated at 10 μg/mL.