CD325 Mouse Monoclonal Antibody

SKU: orb705540

Description

The CD325 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within human CD325. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD325. The exact sequence is proprietary.
• Target: CDH2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325

Western blot analysis of CD325 expression in HepG2 (A), Hela (B), A431 (C) whole cell lysates. (Predicted band size: 99 kD; Observed band size: 110 kD)

Immunohistochemical analysis of CD325 staining in human fallopian tube formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of CD325 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

CDH2

UniProt

P19022

NCBI Gene ID

1000