Anti-CD32 [MDE-9]

SKU: orb1821643

Description

Anti-CD32 [MDE-9]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, ELISA, FC, IF, IHC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: MDE-9
• Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
• Target: CD32
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; FcgammaRIIa-H131; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a

Flow cytometry using the Anti-CD32 antibody MDE-9. Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of MDE-9 (orb1821643, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood leukocytes with Anti-CD32 MDE-9. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes immobilized on Shi-fix™ plus cover-slips stained with the chimeric mouse IgG1 version of MDE-9 (orb1821643) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821643, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD32

UniProt

P12318