Anti-CD32 [AT130-5]

• Catalog Number: orb758762
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD32b
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AT130-5
• Tested applications: Blocking, FC, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: The original antibody was generated by immunizing BALB/c FcγRIIB—/— mice with FcγRIIB-rat CD4 domain 3/4 fusion protein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD32
• UniProt ID: P08101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FcγRIIB; FcgRIIb; CD32B; CDw32; FCG2; FCGR2; FCGR2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-CD32 antibody AT130-5. RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of AT130-5 (orb758763, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD32 antibody AT130-5. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (orb758763) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758763, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD32 antibody AT130-5. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of AT130-5 (orb758763) at 10 μg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue).