CD31 Antibody

SKU: orb1474204

Description

The CD31 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human CD31 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human CD31 protein fragments expressed in E.coli.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Disclaimer: For research use only

Alternative Names

Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31

Western blot analysis of CD31 expression in THP1 (A) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 130 kD)

Immunohistochemical analysis of CD31 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.144). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P16284

NCBI Gene ID

5175