Anti-CD30 [Ki-4]

SKU: orb348920

Description

Rabbit monoclonal antibody to CD30

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: Ki-4
• Immunogen: Human CD30.
• Target: CD30
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

TNFRSF8; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30

Flow-cytometry using anti-CD30 antibody Ki-4 Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Ki-4 (orb348920) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

CD30

UniProt

P28908

References

Horn-Lohrens, O. et al. Shedding of the soluble form of CD30 from the Hodgkin-analogous cell line L540 is strongly inhibited by a new CD30-specific antibody (Ki-4) Int J Cancer, 60, 539-544 (1995)

PubMed: 7530238