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Item 1 of 3
CD30 Biosimilar Antibody
Catalog Number: orb689322
| Catalog Number | orb689322 |
|---|---|
| Category | Antibodies |
| Description | Anti-CD30 (brentuximab biosimilar) mAb |
| Target | CD30 |
| Clonality | Monoclonal |
| Species/Host | Chimeric |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Powder |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | P28908 |
| Tested applications | ELISA, FC |
| Dilution range | ELISA 1:5000-10000 Flow Cyt 1:100 |
| Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Antibody Type | Biosimilar Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TNFRSF8CD30D1S166EKi-1 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Anti-CD30 Neutralizing antibody in a linear range of 0.13-80.0 ng/ml.
Flow cytometry analysis with Anti-CD30 (brentuximab) on Expi293 cells transfected with human CD30 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of anti-human CD30 mAb (orb689322). (A) A clear peak shift of orb689322 was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of orb689322 to CD30. Antibodies were incubated at 2 μg/mL.
