Anti-CD30 (brentuximab biosimilar) mAb

• Catalog Number: orb689322
• Category: Antibodies
• Description: Anti-CD30 (brentuximab biosimilar) mAb
• Species/Host: Chimeric
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Biosimilar Antibody
• Dilution range: ELISA 1:5000-10000; Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: CD30
• UniProt ID: P28908
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFRSF8;CD30;D1S166E;Ki-1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Anti-CD30 Neutralizing antibody in a linear range of 0.13-80.0 ng/ml.

Flow cytometry analysis with Anti-CD30 (brentuximab) on Expi293 cells transfected with human CD30 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human CD30 mAb (orb689322). (A) A clear peak shift of orb689322 was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of orb689322 to CD30. Antibodies were incubated at 2 μg/mL.