Anti-CD3 epsilon [OKT-3 (muromonab)]

• Catalog Number: orb613773
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3E
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OKT-3 (muromonab)
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Human peripheral E+ cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD3 epsilon
• UniProt ID: P07766
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD3e; T-cell surface glycoprotein CD3 epsilon chai
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Molt4 cells with anti-CD3 epsilon antibody OKT-3. Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of OKT-3 (orb613774) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613774, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.