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Anti-CD29 [K20]
Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-Fr |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | K20 |
| Immunogen | Human T-cell lymphoma cells injected into Biozzi high-responder mice. |
| Target | CD29 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Flow-cytometry using anti-CD29 antibody K20 Human leukocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of K20 (orb348910, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Protocol Information
Amiot, M. et al. The human cell surface glycoprotein complex (gp 120,200) recognized by monoclonal antibody K20 is a component binding to phytohaemagglutinin on T cells Scand J Immunol, 23, 109-118 (1986)
Anti-CD29 [K20] (orb348910)
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