TLR8 Antibody

SKU: orb340756

Description

Rabbit polyclonal antibody to TLR8

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human CD288
• Target: TLR8
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Toll-like receptor 8; CD288

Western blot analysis of CD288 expression in THP1 (A), mouse liver (B), mouse brain (C) whole cell lysates. (Predicted band size: 119; 121 kD; Observed band size: 150 kD)

Immunofluorescent analysis of CD288 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

TLR8

UniProt

Q9NR97, P58682

NCBI Gene ID

170744, 51311