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Anti-CD276/B7 H3 Rabbit Monoclonal Antibody
Catalog Number: orb547977
| Catalog Number | orb547977 |
|---|---|
| Category | Antibodies |
| Description | Anti-CD276/B7 H3 Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | ABGB-3 |
| Tested applications | FC, IP, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human CD276 |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 90 kDa |
| UniProt ID | Q5ZPR3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Cos Read more... |
| Note | For research use only |
| Application notes | WB 1:500-1:2000IP 1:50FC 1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD276 expression in 293T cell lysate (orb547977). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 monoclonal antibody (Catalog # orb547977) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CD276.
