Anti-CD27 [RM27-3E5]

• Catalog Number: orb613955
• Category: Antibodies
• Description: Rat monoclonal antibody to CD27
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: RM27-3E5
• Tested applications: FC, IP
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD27
• UniProt ID: P41272
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRS7; CD27 antigen; CD27L receptor; T-cell activ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-CD27 antibody on CD27-Fc fusion protein, with a CD70 (CD27-L) competition assay. Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (orb613954; red line) and isotype control (anti-Fluorescein antibody orb256390 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of orb613954 was used.

ELISA of anti-CD27 antibody on CD27-Fc fusion protein. Binding curves of the human chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (red line) and isotype control (anti-Fluorescein antibody orb256390 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 µg/ml.