Anti-CD257 (BAFF) [Tabalumab]

• Catalog Number: orb1152486
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD257
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Tabalumab
• Tested applications: ELISA, FC, Functional Studies, IF, IP, WB
• Reactivity: Human, Monkey, Rabbit
• Isotype: Human IgG
• Immunogen: Monoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 µg human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD257 (BAFF)
• UniProt ID: Q9Y275
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFSF20; B-cell activating factor; Tumor Necrosis
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of human peripheral blood monocytes with anti-CD257 (BAFF) Tabalumab. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of Tabalumab (orb1152486) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152486, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.