Anti-CD25 [7D4]

• Catalog Number: orb1463357
• Category: Antibodies
• Description: Rat monoclonal antibody to CD25
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 7D4
• Tested applications: Depletion, FC
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: The original rat IgM version of this antibody was raised by immunizing Lewis rats with HT2 cells in complete Freund's adjuvant via a intraperitoneal injection and subsequent generation of hybridomas.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD25
• UniProt ID: P01590
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL-2-RA; Interleukin-2 receptor subunit alpha; IL-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of mouse splenocytes with anti-CD25 7D4. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes stained with the chimeric rabbit IgG version of 7D4 (orb1463357) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463357, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD25 antibody 7D4. Mouse thymus (0.5 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 7D4 (orb1463357) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.