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Anti-CD25 [7D4]

Catalog Number: orb1463357

DispatchUsually dispatched within 5-10 working days
$ 530.00
Catalog Numberorb1463357
CategoryAntibodies
DescriptionRat monoclonal antibody to CD25
TargetCD25
ClonalityMonoclonal
Species/HostRat
IsotypeRat IgG
ConjugationUnconjugated
ReactivityMouse
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% Proclin 300.
PurityPurified
ImmunogenThe original rat IgM version of this antibody was raised by immunizing Lewis rats with HT2 cells in complete Freund's adjuvant via a intraperitoneal injection and subsequent generation of hybridomas.
UniProt IDP01590
Tested applicationsDepletion, FC
Clone Number7D4
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesIL-2-RA; Interleukin-2 receptor subunit alpha; IL-
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NoteFor research use only
Anti-CD25 [7D4]

Immunofluorescence staining of mouse splenocytes with anti-CD25 7D4. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes stained with the chimeric rabbit IgG version of 7D4 (orb1463357) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463357, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Anti-CD25 [7D4]

Western Blot using anti-CD25 antibody 7D4. Mouse thymus (0.5 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 7D4 (orb1463357) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.