Anti-CD23 FCER2 Monoclonal Antibody

• Catalog Number: orb548293
• Category: Antibodies
• Description: Anti-CD23 FCER2 Monoclonal Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human CD23 This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen) .
• UniProt ID: P06734
• MW: 48 kDa
• Tested applications: FC, IHC, WB
• Application notes: WB 1:500-1:1000IHC 1:50-1:200FC 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: ADIC-6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Laminin subunit alpha-3; Epiligrin 170 kDa subunit
• Note: For research use only

Western blot analysis of FCER2 using anti-FCER2 antibody in Raji cell lysate (orb548293). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCER2 antigen affinity purified polyclonal antibody (Catalog # orb548293) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.