Anti-CD23 [CAT-13.9C1]

• Catalog Number: orb758911
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD23
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CAT-13.9C1
• Tested applications: IF
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising mice with with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD23
• UniProt ID: P06734
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Low affinity IgE Receptor; Low affinity immunoglob
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD23 antibody CAT-13.9C1. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of CAT-13.9C1 (orb758911, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD23 antibody CAT-13.9C1. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version CAT-13.9C1 (orb758911) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758911, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.