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Anti-CD222 Antibody

Catalog Number: orb393323

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb393323
CategoryAntibodies
DescriptionRabbit polyclonal antibody to IGF2R.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD222. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetIGF2R
Entrez16004, 3482
UniProt IDQ07113, P11717
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesMPRI; Cation-independent mannose-6-phosphate recep
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CD222 Antibody

Western blot analysis of CD222 expression in Jurkay (A), K562 (B), Caco2 (C) whole cell lysates. (Predicted band size: 274 kD; Observed band size: 274 kD)

Anti-CD222 Antibody

Immunohistochemical analysis of CD222 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD222 Antibody

Immunofluorescent analysis of CD222 staining in Jurkay cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-Hu CD222 Purified [orb44197]

    FC,  IP,  WB

    Human, Primate

    Monoclonal

    Unconjugated

    0.1 mg
  • Anti-Hu CD222 PE [orb44199]

    FC

    Human, Primate

    Monoclonal

    PE

    100 T
  • Anti-Hu CD222 FITC [orb44198]

    FC

    Human, Primate

    Monoclonal

    FITC

    100 T
  • Anti-Hu CD222 APC [orb44200]

    FC

    Human, Primate

    Monoclonal

    APC

    100 T
  • IGF2R (Phospho-Ser2409) Antibody [orb652780]

    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl