Anti-CD22 [hL22 (Epratuzumab)]

SKU: orb411594

Description

Human monoclonal antibody to CD22

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, FC, IP
• Reactivity: Human, Primate

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Human IgG
• Clone No.: hL22 (Epratuzumab)
• Immunogen: This antibody was prepared by the humanization of LL2 (EPB-2), a murine anti-CD22 IgG2a raised against Raji Burkitt lymphoma cells. Murine sequences comprise 5–10% of the molecule, with the remainder being human framework sequences, which greatly reduces the potential for immunogenicity (Traczewski, 2010).
• Target: CD22
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Leu14; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14

Flow-cytometry using anti-CD22 antibody Epratuzumab Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Epratuzumab (orb411594, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD22 antibody Epratuzumab Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Eptratuzumab (orb411594, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD22 antibody Epratuzumab Rhesus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Epratuzumab (orb411594, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

CD22

UniProt

P20273