Anti-CD205 [NLDC145]

• Catalog Number: orb1152485
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD205
• Target: CD205
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising Wistar rats with mouse lymph node stroma.
• UniProt ID: Q60767
• Tested applications: FC, IB, IF, IHC
• Clone Number: NLDC145
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ly-75; NLDC-145; DEC205; DEC-205; Lymphocyte antig
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD205 antibody NLDC145. Paraformaldehyde fixed mouse thymocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of NLDC145 (orb1152485, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse thymocytes with anti-CD205 NLDC145. Immunofluorescence analysis of paraformaldehyde fixed mouse thymocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of NLDC145 (orb1152485) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152485, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.