Anti-CD20 [18B12]

• Catalog Number: orb758830
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD20
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 18B12
• Tested applications: FA, FC, IF, WB
• Reactivity: Mouse
• Isotype: Mouse IgG
• Immunogen: This antibody was generated by immunizing CD20−/− mice with CD20-transfected cell lines (300.18 and 70Z3) and CD20-peptide-keyhole limpet hemocyanin conjugate.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD20
• UniProt ID: P19437
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ly-44; B-cell differentiation antigen Ly-44; B-lym
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-CD20 antibody 18B12. Splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 18B12 (orb758830, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells using anti-CD20 antibody 18B12. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of 18B12 (orb758830) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758830, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.