Anti-CD2 [YTH 655]

• Catalog Number: orb613786
• Category: Antibodies
• Description: Rat monoclonal antibody to CD2
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: YTH 655
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: This antibody was raised against CD2 expressing cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD2
• UniProt ID: P06729
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LFA-2; T-cell surface antigen CD2; Erythrocyte rec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Molt4 cells with anti-CD2 antibody YTH 655. Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YTH 655 (orb613785) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane, and some cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613785, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD2 antibody YTH 655 Human tonsil lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 655 (orb613785) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa, but this protein is glycosylated at several residues. orb613785 successfully detected CD2 in human tonsil lysate.