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Description
Research Area
Immunology & Inflammation
Images & Validation
−| Tested Applications | ELISA, FC, IF, IP, RIA |
|---|---|
| Reactivity | Human, Monkey |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 1B4 |
| Immunogen | Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest. |
| Target | CD18 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; ITGB2; MFI7; IB4; m1B4; mIB4
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Quick Database Links
Gene Symbol
CD18
UniProt
UniProt Details
− No UniProt data available
Protocol Information
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
RIA
Radioimmunoassay
![Anti-CD18 [1B4]](/images/pub/media/catalog/product/NewWebsite/35/orb669756_1.png)
![Anti-CD18 [1B4]](/images/pub/media/catalog/product/NewWebsite/35/orb669756_2.png)
![Anti-CD18 [1B4]](/images/pub/media/catalog/product/NewWebsite/35/orb669757_1.png)
![Anti-CD18 [1B4]](/images/pub/media/catalog/product/NewWebsite/35/orb669757_2.png)