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Anti-CD168 HMMR Rabbit Monoclonal Antibody

Catalog Number: orb548347

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Catalog Numberorb548347
CategoryAntibodies
DescriptionAnti-CD168 HMMR Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberFFA-8
Tested applicationsIHC, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human CD168
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW35 kDa
UniProt IDO75330
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHyaluronan mediated motility receptor; Intracellul
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NoteFor research use only
Application notesWB 1:500-1:2000IHC 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-CD168 HMMR Rabbit Monoclonal Antibody

Western blot analysis of CD168 expression in LnCaP cell lysate (orb548347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMMR monoclonal antibody (Catalog # orb548347) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

Anti-CD168 HMMR Rabbit Monoclonal Antibody

IHC analysis of paraffin-embedded section of human testis using anti-CD168 antibody (orb548347). CD168 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD168 Antibody (orb548347) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.